Characterization of the venom and external morphology of a natural hybrid between Crotalus atrox and Crotalus mictlantecuhtli.

Here we report, for the first time, a natural hybrid between Crotalus atrox and C. mictlantecuhtli based on intermediate characteristics of the external morphology and venom. Morphologically, the individual had characteristics of both parent species. The hybrid's venom exhibited an intermediate composition including the presence of crotoxin which has never been documented in C. atrox but is well documented in C. mictlantecuhtli. The hybrid's venom was highly toxic and showed an intermediate proteolytic activity between the parental species. The two Mexican antivenoms were able to neutralize the hybrid's venom's lethality.

Accessing cryptic diversity in Neotropical rattlesnakes (Serpentes: Viperidae: Crotalus) with the description of two new species.

Members of the Crotalus durissus species complex are widely distributed from Mexico to Argentina in areas with mainly seasonally dry tropical deciduous forest. Although four species (C. culminatus, C. durissus, C. simus and C. tzabcan) are currently recognized, species limits remain to be tested. Previous genetic studies suggest that C. durissus and C. simus may be paraphyletic and that at least one cryptic species may be present. We analyzed 2596 bp of DNA sequence data from three mitochondrial and one nuclear gene to infer phylogenetic relationships in the Neotropical rattlesnakes. We also examined museum and wild specimens as well as captive animals to analyze morphological characters. Our results suggest that the current taxonomy of the Crotalus durissus species complex does not reflect evolutionary history. We found strong support for five independent lineages within Crotalus simus (sensu lato), with genetic and morphological evidence for three previously recognized taxa and two new species, as well as three major lineages within C. durissus that each represent species hypothesis to be tested with additional evidence. We also found support to retain C. totonacus in the Crotalus molossus species complex. We suggest conservative taxonomic changes to the complex and related species, but more evidence is needed (e.g., morphology, ecology and venom composition) to clarify relationships among species.

Description of the male of Craugastor yucatanensis (Lynch, 1965) (Anura, Craugastoridae), its advertisement call, and additional data on females.

The male of Craugastor yucatanensis (Lynch, 1965) is described for the first time, as the original description was based on four females. The advertisement call is described and additional morphological data on females are presented. Also, information is provided on the sexual dimorphism and natural history of the species.

Endoparasites of Crotalus tzabcan (Serpentes: Viperidae), with a checklist in rattlesnakes.

The helminth and pentastomid fauna of 50 specimens of Crotalus tzabcan from the Yucatán Peninsula, Mexico is documented. The examination revealed the presence of three nematode species (Hastospiculum onchocercum, Hexametra boddaertii, and Travassosascaris araujoi), and one pentastomid (Porocephalus crotali). The threee nematode species had the same prevalence (2%), while the pentastomid had a higher prevalence (8%). The pentastomid P. crotali was the most abundant and intense parasite, although it was only found in four snake hosts. Crotalus tzabcan acts as definitive host for the adult helminths and pentastomids, with rodents as the probable intermediate hosts. This work represents the first systematic survey on the parasitic helminth and pentastomid fauna of C. tzabcan, and includes four new geographical records. Additionally, a checklist of helminths and pentastomids reported for Crotalus and Sistrurus is provided. To date, a total of 32 helminth and 7 pentastomid species have been recorded as parasites of rattlesnakes. Nematoda possessed the highest species richness. The genera with the highest number of host species were Mesocestoides and Hexametra, followed by Kalicephalus. The rattlesnake species with the highest number of reported parasites was C. durissus (18 nematodes and 2 pentastomids).